ABclonal

GFAP Rabbit pAb

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  • €59,00
  • Regular price €101,00
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Manufacture

ABclonal

Product name

GFAP Rabbit pAb

Catalog No.

AB-A0237

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1)

Gene ID

2670

Swiss prot

P14136

Synonyms

ALXDRD; GFAP

Calculated MW

49kDa/50kDa

Observed MW

50KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

Mouse brain, Rat brain

Customer validation

(Mouse glial cells, Mouse brain)

WB(Mus musculus)

IF(Mus musculus)

IF(Anatinae, Rattus norvegicus, Mus musculus, Homo sapiens)

PMID: 26166359  Title: Interleukin-17A Acts to Maintain Neuropathic Pain Through Activation of CaMKII/CREB Signaling in Spinal Neurons  PMID: 32063900  Title: Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus  PMID: 30087244  Title: Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes.  PMID: 32784362  Title: TLR Antagonism by Sparstolonin B Alters Microbial Signature and Modulates Gastrointestinal and Neuronal Inflammation in Gulf War Illness Preclinical Model  PMID: 32871241  Title: Myocardial infarction-induced anxiety-like behavior is associated with epigenetic alterations in the hippocampus of rat  PMID: 29106517  Title: TPD52L2 impacts proliferation, invasiveness and apoptosis of glioblastoma cells via modulation of wnt/β-catenin/snail signaling 
ABclonal:Western blot - GFAP Rabbit pAb (A0237) }

Western blot - GFAP Rabbit pAb (A0237)

Western blot analysis of extracts of various cell lines, using GFAP Rabbit pAb (A0237) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237) }

Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GFAP Rabbit pAb (A0237) }

Immunohistochemistry - GFAP Rabbit pAb (A0237)

Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of U-251 MG cells using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of rat brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GFAP Rabbit pAb (A0237) }

Immunofluorescence - GFAP Rabbit pAb (A0237)

Immunofluorescence analysis of mouse brain using GFAP Rabbit pAb (A0237) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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