ABclonal

FoxO3a Rabbit pAb

  • Sale
  • €59,00
  • Regular price €101,00
Shipping calculated at checkout.

* Price changes reserved. Please refer to the price on the confirmation letter.


Product name FoxO3a Rabbit pAb
Catalog No. AB-A0102
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1).

Gene ID

56484

Swiss prot

Q9WVH4

Synonyms

FOXO3; AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A

Calculated MW

71kDa

Observed MW

80-90KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, Jurkat, 293T, PC-3
Cellular location Cytoplasm, Nucleus, cytosol
Customer validation

WB(Danio rerio, Rattus norvegicus, Mus musculus, Homo sapiens)

IP(Rattus norvegicus)

Co-IP(Rattus norvegicus)

PMID: 27777301  Title: Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL)  PMID: 27064125  Title: Activation of SIRT3 attenuates triptolide-induced toxicity through closing mitochondrial permeability transition pore in cardiomyocytes  PMID: 28541289  Title: miR-29b contributes to multiple types of muscle atrophy  PMID: 32032850  Title: Andrographolide attenuates oxidative stress injury in cigarette smoke extract exposed macrophages through inhibiting SIRT1/ERK signaling  PMID: 31100089  Title: Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes.  PMID: 31560953  Title: GL-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.  PMID: 31881011  Title: Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1.  PMID: 32705806  Title: Andrographolide attenuates epithelial‐mesenchymal transition induced by TGF‐β1 in alveolar epithelial cells  PMID: 33316740  Title: Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway 

 

ABclonal:Western blot - FoxO3a Rabbit pAb (A0102) }

Western blot - FoxO3a Rabbit pAb (A0102)

Western blot analysis of extracts of various cell lines, using FoxO3a antibody (A0102) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - FoxO3a Rabbit pAb (A0102) }

Immunohistochemistry - FoxO3a Rabbit pAb (A0102)

Immunohistochemistry of paraffin-embedded Human colon using FoxO3a Rabbit pAb (A0102) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - FoxO3a Rabbit pAb (A0102) }

Immunohistochemistry - FoxO3a Rabbit pAb (A0102)

Immunohistochemistry of paraffin-embedded Mouse kidney using FoxO3a Rabbit pAb (A0102) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - FoxO3a Rabbit pAb (A0102) }

Immunofluorescence - FoxO3a Rabbit pAb (A0102)

Immunofluorescence analysis of C6 cells using FoxO3a Rabbit pAb (A0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FoxO3a Rabbit pAb (A0102) }

Immunofluorescence - FoxO3a Rabbit pAb (A0102)

Immunofluorescence analysis of NIH-3T3 cells using FoxO3a Rabbit pAb (A0102) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

 

[ndnapps-contactform]