Cat. NO. AB-RK20620 (datasheet)
FirstTaq DNA Polymerase is an optimized blend of Taq Klenow and Deep Oean DNA polymerases for use with routine and difficult PCR experiments. The 3'→ 5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. The FirstTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content.
FirstTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~50% GC content, FirstTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the FirstTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the FirstTaq High GC Enhancer can be added at a final concentration of 10-20% to reactions containing FirstTaq GC Reaction Buffer.
|Applications||Multiplex PCR, Specialty PCR, PCR , Routine PCR|
|Unit Definition||One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.|
|Storage Conditions||10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA-630, 50% Glycerol, pH7.4 @ 25°C|
|Reaction Conditions||1X FirstTaq Standard Reaction Buffer|
|Reaction Buffer or Mix||1X FirstTaq Standard Reaction Buffer:
20 mM Tris-HCl, 22 mM NH4Cl , 22 mM KCl, 1.8 mM MgCl2, 0.06% IGEPAL CA-630, 0.05% Tween 20, pH8.9 @ 25°C
|5' - 3' Exonuclease||No|
|3' - 5' Exonuclease||Yes|
|Resulting Ends||Single-base 3' Overhangs|
|Error Rate||< 140x10-6 bases|