FirstTaq 2X PCR Master Mix with Standard Buffer

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Cat. NO. AB-RK20622 (datasheet)

FirstTaq 2X Master Mix with Standard Buffer is an optimized blend of Taq klenow and Deep Ocean DNA Polymerases ideally suited for routine PCR applications using a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3'- 5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. This convenient master mix contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.

Featured Specification
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Storage Temperature -20°C
Reaction Buffer or Mix 1X Master Mix Composition:
20 mM Tris-HCl, 22 mM KCl, 22 mM NH4Cl, 1.8 mM MgCl2, 0.2 mM dNTPs, 25 units/ml FirstTaq DNA Polymerase, 0.06% IGEPAL CA-630, 0.05% Tween 20, 5% Glycerol, pH8.9 @ 25°C
Heat Inactivation No
5' - 3' Exonuclease No
3' - 5' Exonuclease Yes
Resulting Ends Single-base 3' Overhangs
Error Rate < 140x10-6 bases