ABclonal

FirstTaq 2X PCR Master Mix with GC Buffer

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  • Regular price €35,00
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Cat. NO. AB-RK20623 (datasheet)

FirstTaq 2X Master Mix with GC Buffer is an optimized blend of Taq klenow and Deep Ocean DNA Polymerases. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3'-> 5 'exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.

Featured Specification
Applications Multiplex PCR, Specialty PCR, PCR , Routine PCR
Storage Temperature -20°C
Reaction Buffer or Mix 1X Master Mix Composition:
80 mM Tris-SO4, 20 mM (NH4)2SO4, 2 mM MgSO4, 5% DMSO, 0.2 mM dNTPs, 25 units/ml FirstTaq DNA Polymerase, 0.05% Tween 20, 0.06% IGEPAL CA-630, 5% Glycerol, pH9.2 @ 25°C
Heat Inactivation No
5' - 3' Exonuclease No
3' - 5' Exonuclease Yes
Resulting Ends Single-base 3' Overhangs
Error Rate < 140x10-6 bases
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