ABclonal

FGFR1 Rabbit pAb

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Product name FGFR1 Rabbit pAb
Catalog No. AB-A0082
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human FGFR1 (NP_075598.2).

Gene ID

2260

Swiss prot

P11362

Synonyms

FGFR1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FLG; FLT-2; FLT2; HBGFR; HH2; HRTFDS; KAL2; N-SAM; OGD; bFGF-R-1

Calculated MW

6kDa/16kDa/33kDa/55-95kDa

Observed MW

100-120kDa

Reactivity Human, Mouse, Rat
Tested applications WB
Recommended dilution WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-87MG, HepG2, U-251MG, Mouse brain, Rat brain
Cellular location Cell membrane, Cytoplasm, Cytoplasmic vesicle, Nucleus, Single-pass type I membrane protein, cytosol
ABclonal:Western blot - FGFR1 Rabbit pAb (A0082) }

Western blot - FGFR1 Rabbit pAb (A0082)

Western blot analysis of extracts of various cell lines, using FGFR1 antibody (A0082) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
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