ABclonal

FGF2 Rabbit pAb

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Product name FGF2 Rabbit pAb
Catalog No. AB-A0235
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).

Gene ID

2247

Swiss prot

P09038

Synonyms

BFGF; FGF-2; FGFB; HBGF-2; Basic FGF; FGF2

Calculated MW

17kDa/21kDa/22kDa/30kDa

Observed MW

20kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples rat ovary
Cellular location Nucleus, Secreted
Customer validation

WB(Gallus gallus, Oryctolagus cuniculus)

IF(Mus musculus)

IHC(Mus musculus)


PMID: 29487404  Title: Role and regulation of growth plate vascularization during coupling with osteogenesis in tibial dyschondroplasia of chickens  PMID: 32460319  Title: The Differential Expression of Cytokines and Growth Factors After SMILE Compared With FS-LASIK in Rabbits  PMID: 33045372  Title: CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force 

 

ABclonal:Western blot - FGF2 Rabbit pAb (A0235) }

Western blot - FGF2 Rabbit pAb (A0235)

Western blot analysis of extracts of rat ovary, using FGF2 antibody (A0235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - FGF2 Rabbit pAb (A0235) }

Immunohistochemistry - FGF2 Rabbit pAb (A0235)

Immunohistochemistry of paraffin-embedded Mouse kidney using FGF2 Rabbit pAb (A0235) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - FGF2 Rabbit pAb (A0235) }

Immunohistochemistry - FGF2 Rabbit pAb (A0235)

Immunohistochemistry of paraffin-embedded Rat ovary using FGF2 Rabbit pAb (A0235) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - FGF2 Rabbit pAb (A0235) }

Immunohistochemistry - FGF2 Rabbit pAb (A0235)

Immunohistochemistry of paraffin-embedded Human colon using FGF2 Rabbit pAb (A0235) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - FGF2 Rabbit pAb (A0235) }

Immunofluorescence - FGF2 Rabbit pAb (A0235)

Immunofluorescence analysis of C6 cells using FGF2 Rabbit pAb (A0235) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FGF2 Rabbit pAb (A0235) }
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