ABclonal

Cytochrome c Rabbit pAb

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Product name Cytochrome c Rabbit pAb
Catalog No. AB-A0225
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).

Gene ID

54205

Swiss prot

P99999

Synonyms

CYCS; CYC; HCS; THC4; cytochrome c

Calculated MW

11kDa

Observed MW

12kDa

Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse heart, Mouse kidney, Rat kidney
Cellular location Mitochondrion intermembrane space
Customer validation

(rat cells, Mouse N2a, BEL-7402)

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Rattus norvegicus)


PMID: 25169008  Title: Triptolide-induced oxidative stress involved with Nrf2 contribute to cardiomyocyte apoptosis through mitochondrial dependent pathways  PMID: 29949756  Title: Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency.  PMID: 29620221  Title: CT‑1042, a novel anticancer agent, exhibits effects by activating p53 and inhibiting survivin  PMID: 31054430  Title: Synthesis and evaluation of redox-sensitive gonadotropin-releasing hormone receptor-targeting peptide conjugates.  PMID: 31894280  Title: Dexamethasone induces aberrant macrophage immune function and apoptosis  PMID: 30256405  Title: Nerve growth factor prevents arsenic‐induced toxicity in PC12 cells through the AKT/GSK‐3β/NFAT pathway  PMID: 26393405  Title: G3-C12 Peptide Reverses Galectin-3 from Foe to Friend for Active Targeting Cancer Treatment 
ABclonal:Western blot - Cytochrome c Rabbit pAb (A0225) }

Western blot - Cytochrome c Rabbit pAb (A0225)

Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (A0225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - Cytochrome c Rabbit pAb (A0225) }

Immunofluorescence - Cytochrome c Rabbit pAb (A0225)

Immunofluorescence analysis of A-549 cells using Cytochrome c antibody (A0225). Blue: DAPI for nuclear staining.
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