ABclonal

Bcl-2 Rabbit mAb

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  • €69,00
  • Regular price €140,00
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Manufacture

ABclonal

Product name

Bcl-2 Rabbit mAb

Catalog No.

AB-A19693

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human Bcl-2

Gene ID

596

Swiss prot

P10415

Synonyms

Bcl-2; PPP1R50; BCL2

Calculated MW

85kDa/105kDa

Observed MW

50kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IP

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

Mouse testis, Mouse lung, 293T

Customer validation

WB(Sanghuangporus vaninii, Homo sapiens, Mus musculus)

PMID: 32629056  Title: Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 …  PMID: 33391463  Title:  LT-171-861, a novel FLT3 inhibitor, shows excellent preclinical efficacy for the treatment of FLT3 mutant acute myeloid leukemia 
ABclonal:Western blot - Bcl-2 Rabbit mAb (A19693) }

Western blot - Bcl-2 Rabbit mAb (A19693)

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A19693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Bcl-2 Rabbit mAb (A19693) }

Immunohistochemistry - Bcl-2 Rabbit mAb (A19693)

Immunohistochemistry of paraffin-embedded human placenta using Bcl-2 antibody (A19693) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - Bcl-2 Rabbit mAb (A19693) }

Immunoprecipitation - Bcl-2 Rabbit mAb (A19693)

Immunoprecipitation analysis of 200ug extracts of Mouse lung using 3ug Bcl-2 antibody (A19693). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A19693) at a dilition of 1:1000.
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