ABclonal

[KO Validated] p38 MAPK Rabbit pAb

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Manufacture

ABclonal

Product name

[KO Validated] p38 MAPK Rabbit pAb

Catalog No.

AB-A14401

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human p38 MAPK (NP_620581.1)

Gene ID

1432

Swiss prot

Q16539

Synonyms

MAPK14; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; p38 MAPK

Calculated MW

29kDa/34kDa/35kDa/41kDa

Observed MW

41KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF, IP

Recommended dilution

WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

HeLa, BxPC-3, Mouse testis, Rat kidney

Customer Validation

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens)

PMID: 31247479  Title: Regulation of an osteon-like concentric microgrooved surface on osteogenesis and osteoclastogenesis.  PMID: 32270801  Title: Molecular mechanisms of selenium-biofortified soybean protein and polyphenol conjugates in protecting mouse skin damaged by UV-B  PMID: 31048666  Title: Cathepsin C Interacts with TNF-α/p38 MAPK Signaling Pathway to Promote Proliferation and Metastasis in Hepatocellular Carcinoma.  PMID: 32590114  Title: In vitro and in vivo anti-inflammatory effects of different extracts from Epigynum auritum through down-regulation of NF-κB and MAPK signaling pathways  PMID: 32690037  Title: CDK7 inhibitor THZ1 enhances antiPD-1 therapy efficacy via the p38α/MYC/PD-L1 signaling in non-small cell lung cancer  PMID: 33218940  Title: Imperatorin reduces the inflammatory response of atherosclerosis by regulating MAPKs signaling pathway in vivo and in vitro  PMID: 32876895   Title: MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1/p38 MAPK Pathway  PMID: 33095085  Title: Preparation, characterization and pharmacodynamic study on deep second degree burns of total flavonoids composite phospholipids liposome of Oxytropis falcata Bunge 
ABclonal:Western blot - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Western blot - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Western blot analysis of extracts from normal (control) and p38 MAPK knockout (KO) HeLa cells, using p38 MAPK antibody (A14401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Western blot - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (A14401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Immunohistochemistry - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry of paraffin-embedded Human placenta using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Immunohistochemistry - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Immunohistochemistry of paraffin-embedded Rat kidney using p38 MAPK Rabbit pAb (A14401) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Immunofluorescence - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Immunofluorescence analysis of NIH-3T3 cells using p38 MAPK antibody (A14401) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] p38 MAPK Rabbit pAb (A14401) }

Immunoprecipitation - [KO Validated] p38 MAPK Rabbit pAb (A14401)

Immunoprecipitation analysis of 200ug extracts of U-87MG cells, using 3 ug p38 MAPK antibody (A14401). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (A14401) at a dilition of 1:1000.
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