ABclonal

RNase Inhibitor, Mammalian

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Manufacturer: ABclonal

RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.

Product Components
Components Cat. No.
RNase Inhibitor, Mammalian (40, 000 U/ml) AB-RK21401

 

Product Characteristics
RT-PCR, cDNA synthesis, In vitro transcription/translation, Enzymatic RNA labeling reaction, Other applications where the integrity of RNA is important
Product Information
Featured Specification
Product Source An E.coli strain that carries the Ribonuclease Inhibitor gene from Porcine.
Applications Cell-Free Protein Expression, PCR
Unit Definition One unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
Storage Conditions 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol, pH7.6 @ 25°C
Storage Temperature -20°C
Documents
Datasheet

 

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