RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
|RNase Inhibitor, Mammalian (40, 000 U/ml)||AB-RK21401|
Product CharacteristicsRT-PCR, cDNA synthesis, In vitro transcription/translation, Enzymatic RNA labeling reaction, Other applications where the integrity of RNA is important
|Product Source||An E.coli strain that carries the Ribonuclease Inhibitor gene from Porcine.|
|Applications||Cell-Free Protein Expression, PCR|
|Unit Definition||One unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.|
|Storage Conditions||20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol, pH7.6 @ 25°C|