ABclonal

Caspase-3 Rabbit pAb

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Product name Caspase-3 Rabbit pAb
Catalog No. AB-A0214
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Caspase-3 (NP_004337.2).

Gene ID

836

Swiss prot

P42574

Synonyms

CPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3

Calculated MW

31kDa

Observed MW

17kDa/32kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, HeLa, 293T, NIH/3T3, mouse lung, mouse liver
Cellular location Cytoplasm
Customer validation

(Eoma cell, U2OS,Saos-2)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus)

IF(Mus musculus)

PMID: 25506218  Title: Immunoliposome co-delivery of bufalin and anti-CD40 antibody adjuvant induces synergetic therapeutic efficacy against melanoma  PMID: 24962416  Title: Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage  PMID: 24652493  Title: Etanercept reduces neuroinflammation and lethality in mouse model of Japanese encephalitis  PMID: 25806467  Title: The marine metabolite SZ-685C induces apoptosis in primary human nonfunctioning pituitary adenoma cells by inhibition of the Akt pathway in vitro  PMID: 23811531  Title: In vitro antioxidant and antiproliferative effects of ellagic acid and its colonic metabolite, urolithins, on human bladder cancer T24 cells  PMID: 26334131  Title: FoxM1 Directs STAT3 Expression Essential for Human Endometrial Stromal Decidualization  PMID: 26196843  Title: Maresin 1 Inhibits Epithelial-to-Mesenchymal Transition in Vitro and Attenuates Bleomycin Induced Lung Fibrosis in Vivo  PMID: 30975176  Title: Compound α-keto acid tablet supplementation alleviates chronic kidney disease progression via inhibition of the NF-kB and MAPK pathways.  PMID: 31054430  Title: Synthesis and evaluation of redox-sensitive gonadotropin-releasing hormone receptor-targeting peptide conjugates.  PMID: 32200236  Title: Ambient particulate matter triggers dysfunction of subcellular structures and endothelial cell apoptosis through disruption of redox equilibrium and calcium homeostasis  PMID: 31119787  Title: Plin3 protects against alcoholic liver injury by facilitating lipid export from the endoplasmic reticulum  PMID: 31560953  Title: GL-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.  PMID: 31316178  Title: LW-213, a newly synthesized flavonoid, induces G2/M phase arrest and apoptosis in chronic myeloid leukemia.  PMID: 29158805  Title: Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.  PMID: 29441017  Title: 15, 16-Dihydrotanshinone I Inhibits Hemangiomas through Inducing Pro-apoptotic and Anti-angiogenic Mechanisms in Vitro and in Vivo.  PMID: 32860849  Title: Pyridoxine induces monocyte-macrophages death as specific treatment of acute myeloid leukemia  PMID: 32882406  Title: The role and mechanism of action of RNF186 in colorectal cancer through negative regulation of NF-κB  PMID: 33185634  Title: Polycyclic polyprenylated acylphloroglucinols from Garcinia xanthochymus fruits exhibit antitumor effects by inhibition of STAT3 signaling pathway 
ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214) }

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (A0214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214) }

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (A0214) at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Caspase-3 Rabbit pAb (A0214) }

Immunohistochemistry - Caspase-3 Rabbit pAb (A0214)

Immunohistochemistry of paraffin-embedded mouse lung using Caspase-3 Rabbit pAb (A0214) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Caspase-3 Rabbit pAb (A0214) }

Immunofluorescence - Caspase-3 Rabbit pAb (A0214)

Immunofluorescence analysis of L929 cells using Caspase-3 Rabbit pAb (A0214) at dilution of 1:100. Blue: DAPI for nuclear staining.
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