ABclonal

Caspase-12 Rabbit pAb

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Product name Caspase-12 Rabbit pAb
Catalog No. AB-A0217
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Caspase-12 (NP_001177945.2).

Gene ID

100506742

Swiss prot

Q6UXS9

Synonyms

CASP12; CASP-12; CASP12P1

Calculated MW

19kDa/29kDa/38kDa

Observed MW

42kDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, Mouse lung, Mouse ovary, Mouse uterus, Rat lung
Cellular location
Customer validation

WB(Homo sapiens, Sus scrofa, Mus musculus)

IHC(Rattus norvegicus)


PMID: 32200236  Title: Ambient particulate matter triggers dysfunction of subcellular structures and endothelial cell apoptosis through disruption of redox equilibrium and calcium homeostasis  PMID: 32208605  Title: Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota after a Challenge with Deoxynivalenol in Weaned Piglets PMID: 30558299  Title: Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells.  PMID: 31545437  Title: Effects of thymosin β4 on neuronal apoptosis in a rat model of cerebral ischemia?reperfusion injury.  PMID: 32695629  Title: Up-regulation of PERK/Nrf2/HO-1 axis protects myocardial tissues of mice from damage triggered by ischemia-reperfusion through ameliorating endoplasmic reticulum stress  PMID: 33243499  Title: Atmospheric particulate matter impedes autophagic flux by impairing lysosomal milieu and integrity in human umbilical vein endothelial cells (HUVECs) 
ABclonal:Western blot - Caspase-12 Rabbit pAb (A0217) }

Western blot - Caspase-12 Rabbit pAb (A0217)

Western blot analysis of extracts of various cell lines, using Caspase-12 Antibody (A0217) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Caspase-12 Rabbit pAb (A0217) }

Immunohistochemistry - Caspase-12 Rabbit pAb (A0217)

Immunohistochemistry of paraffin-embedded human prostate using Caspase-12 antibody (A0217) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - Caspase-12 Rabbit pAb (A0217) }

Immunohistochemistry - Caspase-12 Rabbit pAb (A0217)

Immunohistochemistry of paraffin-embedded human stomach using Caspase-12 antibody (A0217) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Caspase-12 Rabbit pAb (A0217) }
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