ABclonal

c-Fos Rabbit pAb

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Product name c-Fos Rabbit pAb
Catalog No. AB-A0236
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 211-380 of human c-Fos (NP_005243.1).

Gene ID

2353

Swiss prot

P01100

Synonyms

FOS; AP-1; C-FOS; p55; c-Fos

Calculated MW

28kDa/36kDa/40kDa

Observed MW

55-60KDa

Reactivity Human, Mouse, Rat
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa
Cellular location Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Rattus norvegicus)


PMID: 28842554  Title: CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression  PMID: 30703397  Title: Metformin administration prevents memory impairment induced by hypobaric hypoxia in rats.  PMID: 31344254  Title: Transcriptomes in rat sciatic nerves at different stages of experimental autoimmune neuritis determined by RNA sequencing.  PMID: 32194406  Title: MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI  PMID: 27358292  Title: Identification of ubiquinol cytochrome c reductase hinge (UQCRH) as a potential diagnostic biomarker for lung adenocarcinoma.  PMID: 33316624  Title: The role of FOS-mediated autophagy activation in the indocyanine green-based photodynamic therapy for treating melanoma  PMID: 32764870  Title:  N-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264. 7 Cell Osteoclastogenesis 

 

ABclonal:Western blot - c-Fos Rabbit pAb (A0236) }

Western blot - c-Fos Rabbit pAb (A0236)

Western blot analysis of extracts of HeLa cells, using c-Fos antibody (A0236) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - c-Fos Rabbit pAb (A0236) }

Immunohistochemistry - c-Fos Rabbit pAb (A0236)

Immunohistochemistry of paraffin-embedded human lung cancer using C-FOS antibody (A0236) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - c-Fos Rabbit pAb (A0236) }

Immunohistochemistry - c-Fos Rabbit pAb (A0236)

Immunohistochemistry of paraffin-embedded mouse heart using C-FOS antibody (A0236) at dilution of 1:150 (40x lens).

 

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