ABclonal

alpha smooth muscle Actin Rabbit mAb

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  • €69,00
  • Regular price €140,00
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Manufacture

ABclonal

Product name

alpha smooth muscle Actin Rabbit mAb

Catalog No.

AB-A17910

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

A synthesized peptide derived from human alpha smooth muscle Actin

Gene ID

59

Swiss prot

P62736

Synonyms

AAT6; ACTSA; MYMY5; ACTA2; actin

Calculated MW

42kDa

Observed MW

42kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

RD, Mouse large intestine, Mouse heart, Mouse brain, Rat lung, Rat heart

Customer validation

IHC(Mus musculus)


PMID: 32296329  Title: Calcipotriol Inhibits NLRP3 Signal Through YAP1 Activation to Alleviate Cholestatic Liver Injury and Fibrosis 



ABclonal:Western blot - alpha smooth muscle Actin Rabbit mAb (A17910) }

Western blot - alpha smooth muscle Actin Rabbit mAb (A17910)

Western blot analysis of extracts of various cell lines, using alpha smooth muscle Actin antibody (A17910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - alpha smooth muscle Actin Rabbit mAb (A17910) }

Immunohistochemistry - alpha smooth muscle Actin Rabbit mAb (A17910)

Immunohistochemistry of paraffin-embedded mouse liver using alpha smooth muscle Actin Rabbit mAb (A17910) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910) }

Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910)

Immunofluorescence analysis of smooth muscle of human colon carcinoma using alpha smooth muscle Actin Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910) }

Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910)

Immunofluorescence analysis of mouse colon using alpha smooth muscle Actin Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910) }

Immunofluorescence - alpha smooth muscle Actin Rabbit mAb (A17910)

Immunofluorescence analysis of rat colon using alpha smooth muscle Actin Rabbit mAb (A17910) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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