ABclonal

ALDH1A1 Rabbit pAb

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Manufacture

ABclonal

Product name

ALDH1A1 Rabbit pAb

Catalog No.

AB-A1802

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2)

Gene ID

216

Swiss prot

P00352

Synonyms

ALDH1A1; ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1

Calculated MW

54kDa

Observed MW

50-55kDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC

Recommended dilution

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

A-549, HT-29, K-562, Mouse liver, Mouse lung, Rat lung

Customer validation

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

PMID: 26837383  Title: Quantitative proteomics analysis reveals glutamine deprivation activates fatty acid β-oxidation pathway in HepG2 cells  PMID: 26983598  Title: Comparative Proteomics Analysis Reveals L-Arginine Activates Ethanol Degradation Pathways in HepG2 Cells  PMID: 28507299  Title: The Fatty Acid β-Oxidation Pathway is Activated by Leucine Deprivation in HepG2 Cells: A Comparative Proteomics Study  PMID: 32581380  Title: Reversing a Model of Parkinson's Disease With in Situ Converted Nigral Neurons PMID: 33102941  Title: Bio-inspired chiral self-assemblies promoted neuronal differentiation of retinal progenitor cells through activation of metabolic pathway     
ABclonal:Western blot - ALDH1A1 Polyclonal Antibody (A1802) }

Western blot - ALDH1A1 Polyclonal Antibody (A1802)

Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (A1802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).
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