|Product name||AP2M1 Rabbit mAb|
|Purification method||Affinity purification|
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
|Reactivity||Human, Mouse, Rat|
|Tested applications||WB IF|
|Recommended dilution||WB 1:500 - 1:2000
IF 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence|
|Positive samples||293T, HepG2, U-87MG, Mouse lung, Mouse liver, Mouse brain, Mouse spleen, Rat brain, Rat spleen|
Western blot - AP2M1 Rabbit mAb (A11070)Western blot analysis of extracts of various cell lines, using AP2M1 Rabbit mAb (A11070) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence - AP2M1 Rabbit mAb (A11070)Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit mAb (A11070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.