ABclonal

AP2M1 Rabbit mAb

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Product name AP2M1 Rabbit mAb
Catalog No. AB-A11070
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Immunogen A synthesized peptide derived from human AP2M1
Sequence
Gene ID 1173
Swiss prot Q96CW1
Synonyms AP50; CLAPM1; mu2
Calculated MW 50kDa
Observed MW 50KDa
Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T, HepG2, U-87MG, Mouse lung, Mouse liver, Mouse brain, Mouse spleen, Rat brain, Rat spleen
Cellular location
ABclonal:Western blot - AP2M1 Rabbit mAb (A11070) }

Western blot - AP2M1 Rabbit mAb (A11070)

Western blot analysis of extracts of various cell lines, using AP2M1 Rabbit mAb (A11070) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - AP2M1 Rabbit mAb (A11070) }

Immunofluorescence - AP2M1 Rabbit mAb (A11070)

Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit mAb (A11070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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