Active Caspase-3 Rabbit mAb

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Product name Active Caspase-3 Rabbit mAb
Catalog No. AB-A11021
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Immunogen Recombinant protein of human CASP3
Gene ID 836
Swiss prot P42574
Synonyms CPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3
Calculated MW 31kDa
Observed MW 17KDa
Reactivity Human
Tested applications WB
Recommended dilution WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jukat
Cellular location Cytoplasm
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ABclonal:Western blot - Active Caspase-3 Rabbit mAb (A11021) }

Western blot - Active Caspase-3 Rabbit mAb (A11021)

Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.