|Product name||Alpha-Fetoprotein (AFP) Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) (NP_001125.1).|
|Synonyms||AFPD; FETA; HPAFP; AFP|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WB IF|
|Recommended dilution||WB 1:500 - 1:2000
IF 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence|
|Positive samples||Mouse liver|
Western blot - Alpha-Fetoprotein (AFP) Rabbit pAb (A11013)Western blot analysis of extracts of mouse liver, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (A11013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunofluorescence - Alpha-Fetoprotein (AFP) Rabbit pAb (A11013)Immunofluorescence analysis of human liver cancer using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (A11013) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Alpha-Fetoprotein (AFP) Rabbit pAb (A11013)Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) Rabbit pAb (red, A11013) at dilution of 1:100. Blue: DAPI for nuclear staining.