|Product name||Alkaline Phosphatase (ALPL) Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) (NP_000469.3).|
|Synonyms||ALPL; AP-TNAP; APTNAP; HOPS; TNAP|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WB IHC|
|Recommended dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry|
|Cellular location||Cell membrane, GPI-anchor, Lipid-anchor|
Western blot - Alkaline Phosphatase (ALPL) Rabbit pAb (A1080)Western blot analysis of extracts of HeLa cells, using Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) antibody (A1080) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit pAb (A1080)Immunohistochemistry of paraffin-embedded mouse brain using Alkaline Phosphatase (Alkaline Phosphatase (ALPL)) antibody (A1080) at dilution of 1:100 (40x lens).