ABclonal

AKT1 Mouse mAb

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Product name AKT1 Mouse mAb
Catalog No. AB-A10605
Host species Mouse
Purification method Affinity purification
Isotype IgG

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen A synthetic peptide of human AKT1
Sequence
Gene ID 207
Swiss prot P31749
Synonyms AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW 48kDa/55kDa
Observed MW 56kDa
Reactivity Human
Tested applications WB
Recommended dilution WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples DU145, A-549, HepG2
Cellular location Cell membrane, Cytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

PMID: 29740735  Title: Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis  PMID: 30450569  Title: Long noncoding RNA STXBP5-AS1 inhibits cell proliferation, migration, and invasion via preventing the PI3K/AKT against STXBP5 expression in non-small-cell lung carcinoma.  PMID: 30683918  Title: The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling.  PMID: 32454461  Title: ZNF139/circZNF139 promotes cell proliferation, migration and invasion via activation of PI3K/AKT pathway in bladder cancer 
ABclonal:Western blot - AKT1 Mouse mAb (A10605) }

Western blot - AKT1 Mouse mAb (A10605)

Western blot analysis of extracts of various cell lines, using AKT1 antibody (A10605) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
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