ACBD5 Rabbit pAb

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Product name ACBD5 Rabbit pAb
Catalog No. AB-A10595
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human ACBD5 (NP_663736.2).
Gene ID 91452
Swiss prot Q5T8D3
Synonyms ACBD5
Calculated MW 46kDa/54kDa/58kDa/60kDa
Observed MW 70kDa
Reactivity Human
Tested applications WB
Recommended dilution WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, LO2, Jurkat, A-549, MCF7
Cellular location Peroxisome membrane, Single-pass membrane protein
ABclonal:Western blot - ACBD5 Polyclonal Antibody (A10595) }

Western blot - ACBD5 Polyclonal Antibody (A10595)

Western blot analysis of extracts of various cell lines, using ACBD5 antibody (A10595) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.