|Product name||ApoER2/LRP8 Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
|Immunogen||A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ApoER2/ApoER2/LRP8 (NP_004622.2).|
|Synonyms||LRP8; APOER2; HSZ75190; LRP-8; MCI1|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WB IHC IF|
|Recommended dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence|
|Positive samples||SH-SY5Y, Rat testis|
|Cellular location||Cell membrane, Secreted, Single-pass type I membrane protein|
Western blot - ApoER2/LRP8 Rabbit pAb (A10517)Western blot analysis of extracts of various cell lines, using ApoER2/ApoER2/LRP8 antibody (A10517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence - ApoER2/LRP8 Rabbit pAb (A10517)Immunofluorescence analysis of Human placenta using ApoER2/ApoER2/LRP8 antibody (A10517) at dilution of 1:100. Blue: DAPI for nuclear staining.