ARAP1 Rabbit pAb

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Product name ARAP1 Rabbit pAb
Catalog No. AB-A10466
Host species Rabbit
Purification method Affinity purification
Isotype IgG

The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).
Gene ID 116985
Swiss prot Q96P48
Synonyms ARAP1; CENTD2
Calculated MW 77kDa/127kDa/135kDa/136kDa/160kDa/162kDa
Observed MW 190kDa
Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T, LO2, HeLa, Mouse liver, Rat liver
Cellular location Cell membrane, Cytoplasm, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein
ABclonal:Western blot - ARAP1 Rabbit pAb (A10466) }

Western blot - ARAP1 Rabbit pAb (A10466)

Western blot analysis of extracts of various cell lines, using ARAP1 antibody (A10466) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.