ABclonal

ADAM10 Rabbit pAb

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Product name ADAM10 Rabbit pAb
Catalog No. AB-A10438
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 214-500 of human ADAM10 (NP_001101.1).
Sequence TTSAEKNTCQLYIQTDHLFFKYYGTREAVIAQISSHVKAIDTIYQTTDFSGIRNISFMVKRIRINTTADEKDPTNPFRFPNIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPSGSSGGICEKSKLYSDGKKKSLNTGIITVQNYGSHVPPKVSHITFAHEVGHNFGSPHDSGTECTPGESKNLGQKENGNYIMYARATSGDKLNNNKFSLCSIRNISQVLEKKRNNCFVESGQPICGNGMVEQGEECDCGYSDQCKDECCFDANQPEGRKCKLKPG
Gene ID 102
Swiss prot O14672
Synonyms ADAM10; AD10; AD18; CD156c; CDw156; HsT18717; MADM; RAK; kuz
Calculated MW 49kDa/84kDa
Observed MW 70kDa/105kDa
Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HT-1080, Raji, Jurkat, Mouse kidney, Mouse brain
Cellular location Cell membrane, Endomembrane system, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

PMID: 32272155  Title: Morin exerts protective effects on encephalopathy and sepsis-associated cognitive functions in a murine sepsis model  PMID: 33478552  Title: Suan-Zao-Ren Decoction ameliorates synaptic plasticity through inhibition of the Aβ deposition and JAK2/STAT3 signaling pathway in AD model of APP/PS1 transgenic mice. 
ABclonal:Western blot - ADAM10 Polyclonal Antibody (A10438) }

Western blot - ADAM10 Polyclonal Antibody (A10438)

Western blot analysis of extracts of various cell lines, using ADAM10 antibody (A10438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ADAM10 Polyclonal Antibody (A10438) }

Immunofluorescence - ADAM10 Polyclonal Antibody (A10438)

Immunofluorescence analysis of HeLa cells using ADAM10 antibody (A10438) at dilution of 1:100. Blue: DAPI for nuclear staining.
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