|Product name||ALDH3B1 Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes a member of the aldehyde dehydrogenase protein family. Aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The encoded protein is able to oxidize long-chain fatty aldehydes in vitro, and may play a role in protection from oxidative stress. Alternative splicing results in multiple transcript variants.
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human ALDH3B1 (NP_001025181.1).|
|Synonyms||ALDH3B1; ALDH4; ALDH7|
|Reactivity||Human, Mouse, Rat|
|Recommended dilution||WB 1:500 - 1:2000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||293T, Mouse kidney, Mouse liver, Rat lung|
|Cellular location||Cell membrane, Lipid-anchor|
Western blot - ALDH3B1 Rabbit pAb (A10415)Western blot analysis of extracts of various cell lines, using ALDH3B1 antibody (A10415) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.