ABclonal

Adenosine Deaminase (ADA) Rabbit pAb

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Product name Adenosine Deaminase (ADA) Rabbit pAb
Catalog No. AB-A1019
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA)) (NP_000013.2).
Sequence MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
Gene ID 100
Swiss prot P00813
Synonyms ADA
Calculated MW 40kDa
Observed MW 41kDa
Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples A-549, Jurkat, U-251MG, DU145, Mouse spleen, Mouse intestine, Mouse stomach
Cellular location Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle lumen, Extracellular side, Peripheral membrane protein
ABclonal:Western blot - Adenosine Deaminase (ADA) Rabbit pAb (A1019) }

Western blot - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Western blot analysis of extracts of various cell lines, using Adenosine Deaminase (Adenosine Deaminase (ADA)) antibody (A1019) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019) }

Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Immunofluorescence analysis of C6 cells using Adenosine Deaminase (Adenosine Deaminase (ADA)) antibody (A1019) at dilution of 1:100. Blue: DAPI for nuclear staining.
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