ABclonal

AGL Rabbit pAb

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Product name AGL Rabbit pAb
Catalog No. AB-A10051
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1233-1532 of human AGL (NP_000633.2).
Sequence EGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL
Gene ID 178
Swiss prot P35573
Synonyms AGL; GDE
Calculated MW 172kDa/174kDa
Observed MW 174kDa
Reactivity Human, Mouse
Tested applications WB
Recommended dilution WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, SW620, Mouse heart, Mouse skeletal muscle
Cellular location Cytoplasm
ABclonal:Western blot - AGL Rabbit pAb (A10051) }

Western blot - AGL Rabbit pAb (A10051)

Western blot analysis of extracts of various cell lines, using AGL antibody (A10051) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
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