|Product name||ADAM22 Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 223-400 of human ADAM22 (NP_004185.1).|
|Synonyms||ADAM22; ADAM 22; MDC2|
|Reactivity||Human, Mouse, Rat|
|Recommended dilution||WB 1:1000 - 1:4000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||SH-SY5Y, Mouse brain, Mouse kidney, Mouse heart, Rat kidney, Rat heart|
|Cellular location||Cell projection, Membrane, Single-pass type I membrane protein, axon|
Western blot - ADAM22 Rabbit pAb (A10030)Western blot analysis of extracts of various cell lines, using ADAM22 antibody (A10030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.