ABclonal

ALK Rabbit pAb

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Product name ALK Rabbit pAb
Catalog No. AB-A0766
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human ALK (NP_004295.2).
Sequence VGSGMGTGQRAGSPAAGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARDLLLPPSSSELKAGRPEARGSLALDCAPLLRLLGPAPGVSWTAGSPAPAEARTLSRVLKGGSVRKLRRAKQLVLELGEEAILEGCVGPPGEAAVGLLQFNLSELFSWWIRQGEGRLRIRLMPEKKASEVGREGRLSAAIRASQPRLLFQIFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQ
Gene ID 238
Swiss prot Q9UM73
Synonyms ALK; CD246; NBLST3
Calculated MW 176kDa
Observed MW 185kDa
Reactivity Human, Mouse
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples A-431
Cellular location Cell membrane, Single-pass type I membrane protein
ABclonal:Western blot - ALK Rabbit pAb (A0766) }

Western blot - ALK Rabbit pAb (A0766)

Western blot analysis of extracts of A-431 cells, using ALK antibody (A0766) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766) }

Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of SH-SY5Y cells using ALK antibody (A0766) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766) }

Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of Neuro-2a cells using ALK antibody (A0766) at dilution of 1:200. Blue: DAPI for nuclear staining.
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