|Product name||ALK Rabbit pAb|
|Purification method||Affinity purification|
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human ALK (NP_004295.2).|
|Synonyms||ALK; CD246; NBLST3|
|Tested applications||WB IHC IF|
|Recommended dilution||WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence|
|Cellular location||Cell membrane, Single-pass type I membrane protein|
Western blot - ALK Rabbit pAb (A0766)Western blot analysis of extracts of A-431 cells, using ALK antibody (A0766) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence - ALK Rabbit pAb (A0766)Immunofluorescence analysis of SH-SY5Y cells using ALK antibody (A0766) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence - ALK Rabbit pAb (A0766)Immunofluorescence analysis of Neuro-2a cells using ALK antibody (A0766) at dilution of 1:200. Blue: DAPI for nuclear staining.