ABclonal

SSB Rabbit pAb

  • Sale
  • Regular price €188,00
Shipping calculated at checkout.

* Price changes reserved. Please refer to the price on the confirmation letter.


The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.

Product name SSB Rabbit pAb
Catalog No. AB-A0630
Host species Rabbit
Purification method Affinity purification
Isotype IgG
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).
Sequence KFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ
Gene ID 6741
Swiss prot P05455
Synonyms SSB; LARP3; La; La/SSB
Calculated MW 46kDa
Observed MW 47kDa
Reactivity Human
Tested applications WBIHCIFIP
Recommended dilution WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples Jurkat, LO2, HepG2, 293T
Cellular location Nucleus
ABclonal:Western blot - SSB Rabbit pAb (A0630) }

Western blot - SSB Rabbit pAb (A0630)

Western blot analysis of extracts of various cell lines, using SSB antibody (A0630) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - SSB Rabbit pAb (A0630) }

Immunohistochemistry - SSB Rabbit pAb (A0630)

Immunohistochemistry of paraffin-embedded human breast cancer using SSB Rabbit pAb (A0630) at dilution of 1:300 (40x lens).
ABclonal:Immunohistochemistry - SSB Rabbit pAb (A0630) }

Immunohistochemistry - SSB Rabbit pAb (A0630)

Immunohistochemistry of paraffin-embedded human esophageal cancer using SSB Rabbit pAb (A0630) at dilution of 1:300 (40x lens).
ABclonal:Immunohistochemistry - SSB Rabbit pAb (A0630) }

Immunohistochemistry - SSB Rabbit pAb (A0630)

Immunohistochemistry of paraffin-embedded human lung cancer using SSB Rabbit pAb (A0630) at dilution of 1:300 (40x lens).
ABclonal:Immunohistochemistry - SSB Rabbit pAb (A0630) }

Immunohistochemistry - SSB Rabbit pAb (A0630)

Immunohistochemistry of paraffin-embedded human thyroid cancer using SSB Rabbit pAb (A0630) at dilution of 1:300 (40x lens).
ABclonal:Immunofluorescence - SSB Rabbit pAb (A0630) }

Immunofluorescence - SSB Rabbit pAb (A0630)

Immunofluorescence analysis of U2OS cells using SSB antibody (A0630) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - SSB Rabbit pAb (A0630) }

Immunoprecipitation - SSB Rabbit pAb (A0630)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug SSB antibody (A0630). Western blot was performed from the immunoprecipitate using SSB antibody (A0630) at a dilition of 1:1000.
[ndnapps-contactform]