ABclonal

YES1 Rabbit pAb

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This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.

 

 

Product name YES1 Rabbit pAb
Catalog No. AB-A0628
Host species Rabbit
Purification method Affinity purification
Isotype IgG
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1).
Sequence MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHK
Gene ID 7525
Swiss prot P07947
Synonyms YES1; HsT441; P61-YES; Yes; c-yes
Calculated MW 60kDa
Observed MW 61kDa
Reactivity Human, Mouse
Tested applications WB IHC IF
Recommended dilution WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples BT-474, HepG2, Mouse liver, Mouse kidney, Mouse heart
Cellular location Cell membrane, Cytoplasm, centrosome, cytoskeleton, cytosol, microtubule organizing center
ABclonal:Western blot - YES1 Rabbit pAb (A0628) }

Western blot - YES1 Rabbit pAb (A0628)

Western blot analysis of extracts of various cell lines, using YES1 antibody (A0628) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - YES1 Rabbit pAb (A0628) }

Immunohistochemistry - YES1 Rabbit pAb (A0628)

Immunohistochemistry of paraffin-embedded mouse brain using YES1 antibody (A0628) at dilution of 1:100 (40x lens).
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