ABclonal

Endo G Rabbit pAb

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The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

Product name Endo G Rabbit pAb
Catalog No. AB-A0604
Host species Rabbit
Purification method Affinity purification
Isotype IgG
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2).
Sequence AELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK
Gene ID 2021
Swiss prot Q14249
Synonyms ENDOG
Calculated MW 32kDa
Observed MW 33kDa
Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, MCF7, SW620, Mouse heart, Mouse kidney, Rat heart
Cellular location Mitochondrion
ABclonal:Western blot - Endo G Rabbit pAb (A0604) }

Western blot - Endo G Rabbit pAb (A0604)

Western blot analysis of extracts of various cell lines, using Endo G antibody (A0604) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Endo G Rabbit pAb (A0604) }

Immunohistochemistry - Endo G Rabbit pAb (A0604)

Immunohistochemistry of paraffin-embedded mouse kidney using Endo G antibody (A0604) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Endo G Rabbit pAb (A0604) }

Immunohistochemistry - Endo G Rabbit pAb (A0604)

Immunohistochemistry of paraffin-embedded mouse lung using Endo G antibody (A0604) at dilution of 1:100 (40x lens).
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