This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
|Product name||DYRK1A Rabbit pAb|
|Purification method||Affinity purification|
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 624-763 of human DYRK1A (NP_001387.2).|
|Synonyms||DYRK1A; DYRK; DYRK1; HP86; MNB; MNBH; MRD7|
|Reactivity||Human, Mouse, Rat|
|Recommended dilution||WB 1:500 - 1:1000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||NCI-H460, 293T, K-562, HeLa, Jurkat, Mouse brain, Rat brain|
|Cellular location||Nucleus speckle|
WB(Homo sapiens,Glycyrrhiza uralensis Fisch)
Western blot - DYRK1A Rabbit pAb (A0595)Western blot analysis of extracts of various cell lines, using DYRK1A antibody (A0595) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.