Alkaline Phosphatase (ALPL) Rabbit mAb

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Product name Alkaline Phosphatase (ALPL) Rabbit mAb
Catalog No. AB-A0514
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

Immunogen A synthesized peptide derived from human Alkaline Phosphatase (ALPL) (ALPL)
Gene ID 249
Swiss prot P05186
Calculated MW 57kDa
Observed MW 80KDa
Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2, U-87MG, Mouse liver, Mouse kidney, Rat liver
Cellular location Cell membrane, GPI-anchor, Lipid-anchor
Customer validation

WB(Mus musculus)

PMID: 32929652  Title: ATP-binding cassette g1 regulates osteogenesis via Wnt/β-catenin and AMPK signaling pathways 
ABclonal:Western blot - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514) }

Western blot - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)

Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514) }

Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)

Immunohistochemistry of paraffin-embedded human placenta using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at dilution of 1:100 (40x lens).