ABclonal

Alkaline Phosphatase (ALPL) Rabbit mAb

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Product name Alkaline Phosphatase (ALPL) Rabbit mAb
Catalog No. AB-A0514
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

Immunogen A synthesized peptide derived from human Alkaline Phosphatase (ALPL) (ALPL)
Sequence
Gene ID 249
Swiss prot P05186
Synonyms AP-TNAP; APTNAP; HOPS; TNAP; TNSALP
Calculated MW 57kDa
Observed MW 80KDa
Reactivity Human, Mouse, Rat
Tested applications WB IHC
Recommended dilution WB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2, U-87MG, Mouse liver, Mouse kidney, Rat liver
Cellular location Cell membrane, GPI-anchor, Lipid-anchor
Customer validation

WB(Mus musculus)

PMID: 32929652  Title: ATP-binding cassette g1 regulates osteogenesis via Wnt/β-catenin and AMPK signaling pathways 
ABclonal:Western blot - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514) }

Western blot - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)

Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514) }

Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)

Immunohistochemistry of paraffin-embedded human placenta using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at dilution of 1:100 (40x lens).
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