|Product name||Alkaline Phosphatase (ALPL) Rabbit mAb|
|Purification method||Affinity purification|
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
|Reactivity||Human, Mouse, Rat|
|Tested applications||WB IHC|
|Recommended dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry|
|Positive samples||HepG2, U-87MG, Mouse liver, Mouse kidney, Rat liver|
|Cellular location||Cell membrane, GPI-anchor, Lipid-anchor|
Western blot - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - Alkaline Phosphatase (ALPL) Rabbit mAb (A0514)Immunohistochemistry of paraffin-embedded human placenta using Alkaline Phosphatase (ALPL) (ALPL) Rabbit mAb (A0514) at dilution of 1:100 (40x lens).