ABclonal

ACHA4 Rabbit mAb

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Product name ACHA4 Rabbit mAb
Catalog No. AB-A0350
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Immunogen A synthesized peptide derived from human ACHA4
Sequence
Gene ID 1137
Swiss prot P43681
Synonyms BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4
Calculated MW 70kDa
Observed MW 70kDa
Reactivity Human, Mouse, Rat
Tested applications WB
Recommended dilution WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples C6, SH-SY5Y, Mouse liver, U-251MG, Mouse brain, Rat testis
Cellular location
ABclonal:Western blot - ACHA4 Rabbit mAb (A0350) }

Western blot - ACHA4 Rabbit mAb (A0350)

Western blot analysis of extracts of various cell lines, using ACHA4 Rabbit mAb (A0350) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
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