|Product name||ACHA4 Rabbit mAb|
|Purification method||Affinity purification|
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
|Reactivity||Human, Mouse, Rat|
|Recommended dilution||WB 1:500 - 1:2000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||C6, SH-SY5Y, Mouse liver, U-251MG, Mouse brain, Rat testis|
Western blot - ACHA4 Rabbit mAb (A0350)Western blot analysis of extracts of various cell lines, using ACHA4 Rabbit mAb (A0350) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.