AKT2 Rabbit pAb

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Product name AKT2 Rabbit pAb
Catalog No. AB-A0336
Host species Rabbit
Purification method Affinity purification
Isotype IgG

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.

Immunogen A synthetic peptide of human AKT2
Gene ID 208
Swiss prot P31751
Calculated MW 51kDa/55kDa
Observed MW 65kDa
Reactivity Human, Mouse, Rat
Tested applications WB IF
Recommended dilution WB 1:500 - 1:2000
IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NIH/3T3
Cellular location Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

PMID: 26546510  Title: Celecoxib reverts oxaliplatin-induced neuropathic pain through inhibiting PI3K/Akt2 pathway in the mouse dorsal root ganglion  PMID: 29207171  Title: Ginsenoside Rh2 inhibits human A172 glioma cell proliferation and induces cell cycle arrest status via modulating Akt signaling pathway  PMID: 31030094  Title: Stemona alkaloids suppress the positive feedback loop between M2 polarization and fibroblast differentiation by inhibiting JAK2/STAT3 pathway in fibroblasts and CXCR4/PI3K/AKT1 pathway in macrophages. 
ABclonal:Western blot - AKT2 Rabbit pAb (A0336) }

Western blot - AKT2 Rabbit pAb (A0336)

Western blot analysis of extracts of various cell lines, using AKT2 antibody (A0336).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - AKT2 Rabbit pAb (A0336) }

Immunofluorescence - AKT2 Rabbit pAb (A0336)

Immunofluorescence analysis of HeLa cells using AKT2 antibody (A0336) at dilution of 1:100. Blue: DAPI for nuclear staining.