ABclonal

β-Tubulin Rabbit pAb

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Manufacture

ABclonal

Product name

β-Tubulin Rabbit pAb

Catalog No.

AB-AC008

Host species

Rabbit

Purification method

Affinity purification

Isotype

IgG

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human β-Tubulin (NP_006077.2)

Gene ID

203068

Swiss prot

P07437

Synonyms

CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; Beta Tubulin; TUBB; β-Tubulin

Calculated MW

50kDa

Observed MW

50KDa

Reactivity

Human, Mouse, Rat

Tested applications

WB, IHC, IF

Recommended dilution

WB 1:500 - 1:5000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Positive samples

SH-SY5Y, MCF7, Mouse testis, Mouse brain, Rat testis

Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Crassostrea gigas, Oncorhynchus mykiss, Brachyura)

IHC(Rattus norvegicus)

IF(Mus musculus, Rattus norvegicus)

IF(Homo sapiens)

PMID: 27511050  Title: The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia  PMID: 27774321  Title: Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation  PMID: 28268166  Title: Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis  PMID: 28827310  Title: Isolation and characterization of string-forming female germline stem cells from ovaries of neonatal mice  PMID: 30144801   Title: Activation of neuronal N-methyl-d-aspartate receptor plays a pivotal role in Japanese encephalitis virus-induced neuronal cell damage  PMID: 30779558  Title: Rhein from Rheum rhabarbarum Inhibits Hydrogen-Peroxide-Induced Oxidative Stress in Intestinal Epithelial Cells Partly through PI3K/Akt-Mediated Nrf2/HO-1 Pathways.  PMID: 30785338  Title: Differentially expressed coding and noncoding RNAs in CoCl2-induced cytotoxicity of C2C12 cells.  PMID: 31612057  Title: Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion  PMID: 29171430  Title: Toxic effect of acrylamide on the development of hippocampal neurons of weaning rats  PMID: 27402673  Title: Transcriptional activation and translocation of ancient NOS during immune response PMID: 31962332  Title: The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity  PMID: 31987731  Title: Effect of electroacupuncture on relieving central post-stroke pain by inhibiting autophagy in the hippocampus  PMID: 32181656  Title: Ginsenoside Rg2 Ameliorates High-Fat Diet-Induced Metabolic Disease through SIRT1  PMID: 32368187  Title: Roles of eIF3m in the tumorigenesis of triple negative breast cancer  PMID: 31311594  Title: In vivo tracking on longer retention of transplanted myocardin gene-modified adipose-derived stem cells to improve erectile dysfunction in diabetic rats.  PMID: 31394311  Title: Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.  PMID: 30679505  Title: Usp7 regulates Hippo pathway through deubiquitinating the transcriptional coactivator Yorkie.  PMID: 30952096  Title: Rhein protects against barrier disruption and inhibits inflammation in intestinal epithelial cells.  PMID: 32355287  Title: ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells  PMID:32368855  Title: CM082, a novel angiogenesis inhibitor, enhances the antitumor activity of gefitinib on epidermal growth factor receptor mutant non-small cell lung cancer in vitro and in vivo  PMID: 31209365  Title: Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina  PMID: 28769769  Title: Transplantation of Hematopoietic Stem Cells Promotes Functional Improvement Associated with NT-3-MEK-1 Activation in Spinal Cord-Transected Rats.  PMID: 28726800  Title: Role of Nicotinamide N-Methyltransferase in Dorsal Striatum in Cocaine Place Preference. PMID: 30821058  Title: ISG12a and its interaction partner NR4A1 are involved in TRAIL-induced apoptosis in hepatoma cells.  PMID: 31612057  Title: Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion  PMID: 32621948  Title: Effects of resveratrol on the levels of ATP, 5-HT and GAP-43 in the hippocampus of mice exposed to chronic unpredictable mild stress  PMID: 32666430  Title: Dietary Selenium Promotes Somatic Growth of Rainbow Trout (Oncorhynchus mykiss) by Accelerating the Hypertrophic Growth of White Muscle  PMID: 32778191  Title: Effect of dietary selenium on postprandial protein deposition in the muscle of juvenile rainbow trout (Oncorhynchus mykiss)  PMID: 32892299  Title: Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors  PMID: 32999280  Title: Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf  PMID: 33243860  Title: Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase  PMID: 33262982  Title: Vi4-miR-185-5p-Igfbp3 Network Protects the Brain From Neonatal Hypoxic Ischemic Injury via Promoting Neuron Survival and Suppressing the Cell Apoptosis  PMID: 32266099   Title: mTOR inhibition overcomes primary and acquired resistance to Wee1 inhibition by augmenting replication stress in epithelial ovarian cancers  PMID: 33095085  Title: Preparation, characterization and pharmacodynamic study on deep second degree burns of total flavonoids composite phospholipids liposome of Oxytropis falcata Bunge  PMID: 28476673  Title: Soluble adenylyl cyclase mediates mitochondrial pathway of apoptosis and ATP metabolism in oyster Crassostrea gigas exposed to elevated CO2  PMID: 25912358  Title: CpG ODNs induced autophagy via reactive oxygen species (ROS) in Chinese mitten crab, Eriocheir sinensis 
ABclonal:Western blot - β-Tubulin Rabbit pAb (AC008) }

Western blot - β-Tubulin Rabbit pAb (AC008)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC008) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008) }

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded rat brain using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008) }

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded rat kidney using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008) }

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry of paraffin-embedded mouse kidney using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008) }

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008) }

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008) }

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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