Western blot analysis of extracts of various cell lines, using ZIC3 antibody (A9476) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||ZIC3 Rabbit pAb|
|Description:||This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZIC3 (NP_003404.1).|
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