Western blot analysis of extracts of various cell lines, using ZFPM2 antibody (A9868) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 40s.
|Product Name:||ZFPM2 Rabbit pAb|
|Description:||The zinc finger protein encoded by this gene is a widely expressed member of the FOG family of transcription factors. The family members modulate the activity of GATA family proteins, which are important regulators of hematopoiesis and cardiogenesis in mammals. It has been demonstrated that the protein can both activate and down-regulate expression of GATA-target genes, suggesting different modulation in different promoter contexts. A related mRNA suggests an alternatively spliced product but this information is not yet fully supported by the sequence.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 982-1151 of human ZFPM2 (NP_036214.2).|
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