Western blot analysis of extracts of various cell lines, using YES1 antibody (A0628) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
|Product Name:||YES1 Rabbit pAb|
|Description:||This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human YES1 (NP_005424.1).|
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