Western blot analysis of extracts of various cell lines, using Tyrosine Hydroxylase antibody (A0028) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
|Product Name:||Tyrosine Hydroxylase Rabbit pAb|
|Description:||The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.|
|Immunogen:||A synTyrosine Hydroxylase etic peptide corresponding to a sequence wiTyrosine Hydroxylase in amino acids 1-100 of human Tyrosine Hydroxylase (NP_954986.2).|
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