Western blot analysis of extracts of SH-SY5Y cells, using TSC2 antibody (A0492) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
|Product Name:||TSC2 Rabbit pAb|
|Description:||Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 900-1150 of human TSC2 (NP_000539.2).|
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