Western blot analysis of extracts of various cell lines, using TSC1 antibody (A0720) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||TSC1 Rabbit pAb|
|Description:||This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 550-650 of human TSC1 (NP_000359.1).|
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