Western blot analysis of extracts of various cell lines, using TDO2 antibody (A13182) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
|Product Name:||TDO2 Rabbit pAb|
|Description:||This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 21-260 of human TDO2 (NP_005642.1).|
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