Western blot analysis of extracts of various cell lines, using Smad6 antibody (A0579) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
|Product Name:||Smad6 Rabbit pAb|
|Description:||The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila ‘mothers against decapentaplegic’ (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 257-496 of human Smad6 (NP_005576.3).|
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