Western blot analysis of extracts of various cell lines, using RIG-I / DDX58 antibody (A0550) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).
|Product Name:||RIG-I / DDX58 Rabbit pAb|
|Description:||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 830 to the C-terminus of human RIG-I / DDX58 (NP_055129.2).|
Help & Support