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Western blot analysis of extracts of various cell lines, using RGS14 antibody (A9962) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat lung using RGS14 Rabbit pAb (A9962) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using RGS14 Rabbit pAb (A9962) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using RGS14 Rabbit pAb (A9962) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using RGS14 Rabbit pAb (A9962) at dilution of 1:100 (40x lens).
RGS14 Rabbit pAb
Catalog No：AB-A9962
Manufacturer：ABclonal
Synonyms：RGS14
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | RGS14 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 230-510 of human RGS14 (NP_006471.2). |
| Gene ID: | 10636 |
| Swiss prot: | O43566 |
| Molecular weight: | 21kDa/36kDa/44kDa/61kDa |
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