Western blot analysis of extracts of various cell lines, using RGMB antibody (A12859) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
|Product Name:||RGMB Rabbit pAb|
|Description:||RGMB is a glycosylphosphatidylinositol (GPI)-anchored member of the repulsive guidance molecule family (see RGMA, MIM 607362) and contributes to the patterning of the developing nervous system (Samad et al., 2005 [PubMed 15671031]).[supplied by OMIM, Apr 2009]|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 210-453 of human RGMB (NP_001012779.2).|
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