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Western blot analysis of extracts of various cell lines, using RASA4 antibody (A9860) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RASA4 Rabbit pAb
Catalog No：AB-A9860
Manufacturer：ABclonal
Synonyms：RASA4;CAPRI;GAPL
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | RASA4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human RASA4 (NP_008920.5). |
| Gene ID: | 10156 |
| Swiss prot: | O43374 |
| Molecular weight: | 85kDa/90kDa |
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